Journal: Cell

Article Title: Friction Mediates Scission of Tubular Membranes Scaffolded by BAR Proteins

doi: 10.1016/j.cell.2017.05.047

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Article Snippet: 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PS) , Avanti Polar Lipids , cat# 810198C.

Techniques: Recombinant, Modification, Electron Microscopy, Software

Journal: American journal of hematology

Article Title: A novel missense variant in ATP11C is associated with reduced red blood cell phosphatidylserine flippase activity and mild hereditary hemolytic anemia.

doi: 10.1002/ajh.27088

Figure Lengend Snippet: FIGURE 2 No obvious membrane lipid change is seen in the patient RBCs when normalized to controls, except a decrease of PC species and differential modulation of PS and PE species based on length and unsaturation. (A) Lipid species were assessed by HPLC-MS on washed, lysed, and lipid-extracted RBCs. Results are expressed as percentage of controls. Data in patient are means of three independent lysates from one experiment (red columns) and data in controls are means ± SD of three different donors each in triplicates (gray columns). (B) Cholesterol content determined through a fluorescent assay kit. Cholesterol content was normalized to Hb content and expressed as percentage of control RBCs. Mean ± SD of eight determinations. Cer, ceramide; ns, not significant; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; RBC, red blood cell; SM, sphingomyelin. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Flippase activity was determined by measuring PS internalization on purified RBCs using flow cytometry, in accordance with previously reported methods.18 Basal flippase-independent flipping activity was determined after treating RBCs with 5 mM N-ethylmaleimide (NEM; Sigma-Aldrich) in PBS supplemented with glucose 0.2% (PBS-G) for 20 min at 37°C, which irreversibly and non-specifically inactivates flippases.22 1 μL of 1 mg/mL fluorescent PS (16:0–06:0 NBD-PS; Avanti Polar Lipids) was added to 1 mL of purified RBCs incubated in PBS-G at a hematocrit of 5% and incubated for 0–20 min at 37°C.

Techniques: Membrane, Fluorescence, Control

Journal: iScience

Article Title: PDZD8-deficient mice accumulate cholesteryl esters in the brain as a result of impaired lipophagy

doi: 10.1016/j.isci.2022.105612

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Article Snippet: NBD-PS (16:0–12:0) , Avanti , Cat#810193C.

Techniques: Produced, Recombinant, Modification, Transfection, Mutagenesis, Software, Microscopy, Blocking Assay, Real-time Polymerase Chain Reaction